توسعه و ارزیابی یک سامانه بینی الکترونیکی بر پایه حسگرهای نیمه‌هادی اکسید فلزی جهت تشخیص و جداسازی اسانس‌های لیمو

اسانس‌ها یا روغن‌های فرار ازجمله مواد موجود در گیاهان هستند که شامل مخلوط پیچیده‌ای از مواد شیمیایی آلی مثل ترپینوئیدها، آلدئیدها، الکل‌ها، استرها، ستن‌ها و غیره می‌باشند. اسانس‌ها از تقطیر مواد فرار موجود در اندام‌های مختلف گیاهان تازه یا خشک به‌دست می‌آیند و وزن مخصوص آن‌ها غالباً از آب کمتر است. اسانس‌ها را می‌توان به سه دسته طبیعی، مشابه طبیعی و مصنوعی تقسیم کرد. روش‌های متداول شناسایی و ارزیابی اسانس‌های روغنی دارای نقطه‌ضعف‌هایی هستند. در این تحقیق یک سامانه بینی الکترونیکی شامل هفت حسگر نیمه‌هادی اکسید فلزی برای تشخیص و تفکیک هشت نوع اسانس لیموی تجاری توسعه داده‌شده و با استفاده از روش‌های تحلیل مولفه‌های اصلی، تحلیل تفکیک خطی و شبکه عصبی مصنوعی ارزیابی شد. بر اساس نتایج حاصل از تحلیل داده‌های این سامانه، روش تحلیل مولفه‌های اصلی با دو مولفه اصلی pc1 و pc2 توانست 99 درصد از واریانس داده‌ها را پوشش دهد. همچنین تمامی حسگرها ضرایب لودینگ بالایی را از خود نشان دادند. روش‌های تحلیل تفکیک خطی و شبکه عصبی مصنوعی نیز به‌ترتیب با دقت بالای 98% و91% قادر به جداسازی نمونه‌ها بودند. بنابراین سامانه بینی الکترونیکی پیشنهادشده نشان داد که ابزار قابل‌اعتماد و کم‌هزینه‌ای جهت جداسازی اسانس‌های لیموی تجاری می‌باشد.

Development and Evaluation of an Electronic Nose System Based on MOS Sensors to Detect and to Distinguish Lemon Essential Oils

Introduction;Essences or essential oils are aromatic compounds that are found in different organs of the plants. Essences can be classified into three groups of natural, synthetic and natural like. Most of the methods that are used to detect and to distinguish essential oils are based on chromatographic methods. However, these analytical methods are time consuming and require expert operators to work with required devices. Moreover, it is necessary to prepare the samples. An electronic nose is known as a tool for mimicking the sense of smell. This tool usually consists of an array of sensors which are used to identify and to isolate a variety of complex odors at a low cost. Since there has been no research on the usage of an electronic nose system for detection and separation of essential oils, the purpose of this study is to develop and to evaluate an electronic nose system for identification and classification of various types of commercial lemon essential oils (synthetic types).;Materials and Methods ;The proposed system consists of a sensor chamber, a sample chamber, an array of MOS sensors, electro valves, a pump, a data acquisition cart and, a processor. Essential oils used in this study includes   eight types of synthetic commercial lemon essential oils that were prepared by ((Avishan Khane Tabiat Sabz)) Company located in chemistry and chemical engineering research center of Iran. One gram sample of each essential oil was prepared to be placed in the sample chamber. Each experiment was carried out in 9 replicates and in three stages of 1 Baseline correction (250 s) 2 Sample smell injection (400 s) and 3 Sensors chamber cleaning (200 s). Data received from the sensors signals were initially preprocessed and normalized and then three methods of principal component analyses (PCA), linear discriminant analyses (LDA) and artificial neural network (ANN) were used to process the data. Both PCA and LDA methods were run using the Unscramble x10.4 software and the artificial neural network was used with the help of NeuroSolution 5 software. In ANN, the classification was carried out using a multilayer perceptron (MLP) and Leaveoneout technique. Leaveoneout is an acceptable method for evaluating the performance of the classification algorithms when the number of samples is low.;Results and Discussion;In order to analyze the data obtained from the sensor array, first, the principal components analysis (PCA) method was used. In this method, the first two principal components of PC 1 and PC 2 totally covered 99% of the data variance. Another plot called as loading plot was used to determine the effects of each sensor responses in pattern recognition analyzes. According to this plot, all sensors had high loading coefficients. In case of distinguishing the lemon essential oils, the results of the linear discriminant analysis (LDA) method showed that this method can distinguish eight types of lemon essential oils with an accuracy of %98. The artificial neural network (ANN) also separated the essential oils with the accuracy of the above %91.;Conclusions ;An Electronic nose system based on semiconductor metal oxide sensors is a powerful tool that can be used as a substitute for traditional methods. In general, this study showed that the electronic nose system based on MOS sensors has the ability to detect and to distinguish commercial lemon essential oils. Considering the wide ranges and economical nature of the essential oils, it is suggested that applications of the electronic nose can be more expanded in the subject of the essential oils of different products.