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   in vitro propagation of damask rose using the temporary immersion system  
   
نویسنده bosh ali ,moieni ahmad ,dehghani hamid ,movahedi zahra
منبع journal of plant physiology and breeding - 2016 - دوره : 6 - شماره : 2 - صفحه:9 -18
چکیده    for centuries, damask rose has been one of the most popular species of the rose family. damask rose can be propagated using vegetative methods such as micropropagation. in this research, the use of temporary immersion system was reported for the first time for damask rose micropropagation. for this reason, a traditional temporary immersion system was designed to facilitate its micropropagation. to optimize this system, the effects of the bap concentration, immersion time, explant size, number of explants per temporary immersion unit were investigated in the independent experiments using completely randomized design. temporary immersion system was also compared with the solid and liquid media. the highest shoot number per explant was obtained from a temporary immersion system using an immersion time of 3 min every 150 min (11.8 shoots), a modified ms medium with 6 mg l^-1 bap (12.33 shoots) and explants with shoot length of 4 cm (12.45 shoots). results indicated that the temporary immersion system was significantly better than solid and liquid media for the shoot number per explant (10.66, 2.56 and 5.66, respectively).
کلیدواژه bap; explant; immersion time; micropropagation; rosa damascena mill
آدرس tarbiat modares university, department of agricultural biotechnology, ایران, tarbiat modares university, faculty of agriculture, department of plant breeding and biotechnology, ایران, tarbiat modares university, faculty of agriculture, department of plant breeding and biotechnology, ایران, malayer university, faculty of agriculture, department of agronomy and plant breeding, ایران
 
 

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