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   artificial polyploidy in the improvement of horticultural crops  
   
نویسنده miri mehdi
منبع journal of plant physiology and breeding - 2020 - دوره : 10 - شماره : 1 - صفحه:1 -28
چکیده    polyploids are organisms that have more than two paired homologous sets of chromosomes. polyploidy has played a significant role in the evolution and diversification of higher plants. artificial polyploidization has been induced using a few antimitotic chemicals such as colchicine, trifluralin and oryzalin. the type of mitotic inhibitors and their concentration and duration time are variable and species-dependent. this technique can be applied ex vitro or in vitro of which, tissue culture is simpler and more efficient. changes in nuclear dna content, gene expression and developmental processes due to ploidy manipulation can lead to morphological, anatomical and physiological changes in polyploid plants. in general, polyploid plants exhibit larger organs, greater biomass, higher yield, superior tolerance to biotic and abiotic stresses as well as higher primary and secondary metabolites. also, polyploidy often reduces fertility and allows the production of seedless fruits. in ornamental plants, increasing the size of polyploid flowers is aesthetically and economically important. there are two direct and indirect methods for the ploidy determination of plants. indirect methods are simpler but more inaccurate, involving the relationship between ploidy level and morphological (i.e. plant height, leaf size and pollen diameter) and anatomical (i.e. stomatal frequency and size as well as chloroplast number in guard cells) properties. in contrast, direct assay methods, such as chromosome counting in mitotic cells of root-tips and flow cytometry are accurate techniques for the determination of ploidy level in plants. overall, polyploidy manipulation has long been used in improving the yield of many crop plants and can be considered as one of the most promising tools in plant breeding programs.
کلیدواژه antimitotic agent ,flow cytometry ,genome doubling ,plant breeding ,polyploid
آدرس islamic azad university, karaj branch, department of horticulture, iran
پست الکترونیکی smmiri@kiau.ac.ir
 
 

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