Development of antibody-based microarray assay for quantitative detection of aflatoxin B1

Background: aflatoxin b1 (afb1) is a toxic metabolite produced by aspergillus species that contaminates a wide range of agricultural products. objectives: this study was designed to develop a rapid and highly sensitive immunoassay method in microarray format for quantitative detection of afb1 to evaluate the potential of microarray platform for high-throughput screening, which can be beneficial in food and feed industry. methods: following successful optimization, using an indirect competitive immunoassay in dot blot format, afb1-bovine serum albumin (afb1-bsa) conjugate was contact-printed onto 16 isolated sub-arrays on multi-pad nitrocellulose coated slides; subsequently used in competitive binding assays. results: using the aforemention-ed assay, afb1 was determined from 15 pg/g to 3.04 ng/g working range with detection limit (lod) of 1 pg/g. to evaluate assay performance in real food matrices, the authors spiked wheat samples with different concentration of afb1. after extraction, working ranges of 0.11-4.15 ng/g with detection limit of 30pg/g was determined. good recoveries (94±9%) were obtained, demonstrat-ing accurate detection of afb1 concentrations in wheat samples. assay procedure completed in 3 hours. conclusions: the results indicated that the proposed developed assay in microarray format could be used for rapid and sensitive detection of afb1in wheat samples.