Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene

Background: the use of igys in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. objectives: development of antibodies against extra cellular domain of influenza m2 (m2e) protein in egg yolk of laying hens. methods: a fusion construct harboring c-terminal of bovine heat shock protein 70 (hsp70) and influenza m2e coding genes was injected to laying hens. serum and egg yolk antibodies were screened for the presence of anti-m2e antibodies by indirect enzyme-linked immunosorbent assay (elisa). results: anti-m2e antibodies were detected in egg yolks and sera of injected hens from 13 and 7 days post injection (pi), with the peak titer detected on 41 and 35 days pi, respectively. conclusions: anti-m2e igy titers could be an index for expression potential of pcdna3.1-m2e-hspc-terminal construct in laying hens. this construct could be considered as a promising tool in production of anti-m2e polyclonal, monospecific igy antibodies. such anti-m2e antibodies could be exploited for influenza diagnostic and therapeutic measures.