mito-tempo protects buck's semen quality during the cooling process

Background: membrane lipid peroxidation is one of the most significant chemical stresses in sperm. however, the utilization of antioxidants in semen diluent protects cells against oxidative damage, which could be a beneficial way to preserve semen quality during the storage process. objectives: the current study aimed to assess the effect of mito-tempo on quality parameters of buck semen during the cooling process. methods: in this experiment, 5 saanen bucks were used, and their semen samples were collected twice a week. after semen collection and primary evaluation, semen samples were diluted and assigned into 5 equal aliquots. then, the samples were added to extenders containing 0, 1, 10, 100, and 1000 μm mito-tempo (triphenylphosphonium chloride). the prepared samples were stored at 5 °c for up to 60 h. chilled sperm motility, progressive motility (pm), viability, mitochondrial activity, membrane functionality, and malondialdehyde (mda) concentration were analyzed during 0, 30, and 60 h. results: in results at time 0, no difference was observed among groups, but at 30 and 60 h storage, higher (p≤0.05) sperm total motility (tm), pm, mitochondrial activity, membrane functionality, viability, and lower lipid peroxidation were observed in 10 and 100 μm mito-tempo groups than the others.conclusion: in conclusion, supplementation of buck cooling medium with mito-tempo as a novel mitochondria-targeted antioxidant could be an impressive method to conserve buck's semen quality during the cooling storage process.