preservation of buck semen quality during chilling storage using coenzyme q10

Background: storing spermatozoa in a cold environment decreases spermatozoa metabolism while maintaining sperm fertility. because of their unique physiological characteristics, sperm cells’ reproductive ability in small ruminants is reduced by cooling.objectives: this study aimed to assess the impact of supplementing chilling medium with coenzyme q10 (coq10) on the quality parameters of buck’s semen after chilling at 4°c.methods: semen was collected, diluted, and divided into five groups with different coq10 supplementation concentrations (0, 1, 2, 5, and 10 µm coq10). collected semen were stored at 4°c for 50 h. the motility, mitochondrial activity, membrane integrity, viability, and lipid peroxidation were measured after 0, 25, and 50 h of chilling.results: according to the findings, different concentrations of coq10 had no impact (p>0.05) on semen at time 0 of storage. after 25 and 50 h of storage, supplementing the chilling medium with 5 µm coq10 increased (p≤0.05) progressive motility (pm), total motility (tm), viability, mitochondrial activity, and membrane integrity compared to other groups. furthermore, 5 µm coq10 produced less lipid peroxidation (p≤0.05) after 25 and 50 h than the other groups.conclusion: adding 5 µm coq10 to the buck’s semen cooling medium is an effective procedure for protecting the quality of buck spermatozoa during cooling storage.