The in vitro Effect of Doxorubicine-G2-FA Treatment on Breast Cancer

Background: to achieve delivery of a drug to tumors, folic acid (fa) was used as a targeting ligand to change nanocarriers. since the folate receptor has more expression in several tumor types such as breast cancer. objectives: the present study aimed to evaluate the effect of doxorubicine-g2-fa (dox- g2-fa) with in vitro assays. the abbreviation of g2 represents the second generation of dendrimer synthesis. methods: for this purpose, dox-g2-fa was synthesized and mass spectroscopy was used to confirm the synthesized component. also, mtt assay, flow cytometry, and gene expression assay by real-time pcr were used to evaluate cell viability, apoptosis, and necrosis. results: in this study, the effect of dox and dox-g2-fa on the expression of bax, bcl2 genes showed that there was a significant decrease in the expression of the bcl2 gene in the dox-g2-fa group compared to dox and control groups(p<0.05). also, the results of flow-cytometry showed that apoptosis in the presence of dox-g2-fa was greater than in the dox group (p<0.05). conclusions: therefore, it seems that the effect of dox-g2-fa on apoptosis is better than the effect of dox usage alone.