evaluate toxocara canis excretory-secretory antigens in experimental allergic encephalomyelitis (eae)

Background: toxocara canis is the most prevalent intestinal roundworm of canid species. objectives: this study aims to evaluate the effects of toxocara canis excretory- secretory antigens (tces ag) on modulating the immune system in experimental allergic encephalomyelitis (eae) model. methods: adult worms of t.canis were collected from dogs to obtain excretory-secretory antigens. female c57bl/6 mice were divided to four groups (5 mice in each) including: group 1 (mog +tces ag), 2 (mog), 3 (normal) and 4 (tces ag). eae was induced in groups 1 and 2 using myelin oligodendrocyte glycoprotein peptide. before eae induction, tces ag was injected in group 1. twenty-nine after eae induction, mice spleens were removed. mononuclear cells were cultured and used for rna extraction.real time pcr was performed to evaluate rna expression levels of t-bet (th1 lineage-specific transcription factor), gata-3 (th2 transcription factor), and foxp3 (treg transcription factor). results: our results indicated the clinical signs (disability score) of mice in group 1 were decreased significantly as compared to the control group. gene expression of t-bet in the tces ag treatment group were noticeably diminished compared to mog and normal group. the expression of gata-3 gene in group 1 was lower than that in group 2. conclusions: it seems that the tces ag may reduce disability score in multiple sclerosis eae model, and other recombinant antigens should be examined.