The Effect of Ligation of the Ovarian Artery onOvarian Follicular Function in Rats

In the present study was performed to evaluate the follicular growth and hormonalchanges after ovarian artery ligation in rats. fifty six virgin 90-day–old female rats were used.the rats were chosen from 100 female rats by a vaginal smear, which showed that they weresynchronized in estrous phase of the menstrual cycle. animals were kept in seven cages (n=8 ineach) at a temperature of 22 ± 2°c and 12/12 hr light/dark conditions. the rats were divided intoa treatment and control groups. on days 8, 14, 21, 30 and 60, after ligation of the right ovarianartery by laparotomy, nine rats were sacrificed for each test group on mentioned days and bothovaries were collected from each rat. histological studies were the carried out on the ovaries.the ovaries on the ligated side had a significantly lower rate of ovulations (p 0.01) than theovaries in the control group on the intact side. the right-sided ovary had small pea–sizedfollicles, which indicated polycystic ovarian syndrome, but no polycystic follicles wereobserved on the intact side and in the control group ovaries. there were no corpora lutea orcorpora albicantia within the ovary subjected to ovarian artery ligation, which indicated thatthere had not been any recent ovulation. furthermore, there was a greater degree of atresia in theligated ovary than the control group. however, no abnormalities were detected in the leftovaries of the experimental group. blood sampling was performed directly from the heart forhormonal analyses on days 5, 10, 20, 30, 40, 50 and 60 after ovarian artery ligation. in theexperimental group, the serum analyses showed a significant (p<0.01) increase in the levels ofestrogen and luteinizing hormone (lh), while the levels of progesterone and follicularstimulating hormone (fsh) decreased by the time in serum of the test group. glucose levelswere measured and found to be increased, as is seen in polycystic ovarian syndrome, whichsuggested the presence of insulin resistance.