SIMULTANEOUS EFFECTS OF SHAKING AND TEMPERATURE ON VEROTOXINI PHAGE INDUCTION FROM VEROTOXIGENIC ESCHERICHIA COLI STRAINS

Induction of lambda phage earring vcroroxin i gene from a verotoxigcnic strains of escherichia coli and released verotoxin l were studied under environmental factors of shaking and termperature. verotoxin iphage in escherichia coli pa 101 and transductants was confirmed by bacteriophage detection assay.shaking of culture media and increasing temperature until 42 degree c increased phage particles in supernatants of escherichia coli pa 101. our results indicate that environmental factors such as shaking movements in natural inhabitares of bacteria such as river or sewage streams and temperature rise in summer season could be factors in induce and release free vcrotoxin i - producing phage particles in nature that in turn could be the source of phage spreading to other related bacteria. and responsible for increased outbreaks of food borne diseases with verotoxigenic escherichia coli in warm monthcs of year in tropical areas.