SOLID PHASE MICROEXTRACTION FOR TRACE ANALYSIS OF URINARY BENZENE IN ENVIRONMENTAL MONITORING

Conventional analytical method for organic pollutants in water requires extraction of the pollutants, usinghazardous solvent. solid phase microextraction is a solvent free equilibrium extraction method, in which,proper calibration can allow quantitative determinations of organic pollutants at a very good sensitivitywithout the use of any organic solvent. because individual volatile organic carbons are generally exposedenvironmentally and present in urine only at trace levels, a sensitive and accurate determination techniqueis essential. so, this study describes the optimization of headspace solid phase microextraction (hs-spme)followed by gc-fid for benzene in spiked urine. through this investigations, the parameters affecting theextraction and gas chromatographic determination of analytes, including extraction time, temperature,desorption temperature, desorption time, salt addition, sample ph, sample volume and sample agitationwere studied. an optimized headspace extraction was carried out at 30°c for 6 min in the presence of 0.2g/ml of nacl in the sample solution. desorption of the analytes was carried out for 60 sec. at 250°c. theoptimized procedure was also validated with three different pools of spiked urine samples and showed agood reproducibility over six consecutive days as well as six within-day experiments. the accuracy,linearity, detection limits were also determined. the headspace solid phase microextraction, gc-fidtechnique provides a relatively simple, convenient, practical procedure, which was here successfullyapplied to determine benzene in spiked urine.