Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil

Petroleum based products are the major source of energy for industries and daily life. leaks and accidental spillsoccur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleumproducts. in the present study we isolated the bacteria from diesel contaminated soil and screened them fordiesel biodegradation capacity. one monoculture isolate identified by 16s rrna gene sequence analysis to beacinetobacter baumannii was further studied for diesel oil biodegradation. the effects of various culture parameters(ph, temperature, nacl concentrations, initial hydrocarbon concentration, initial inoculum size, role of chemicalsurfactant, and role of carbon and nitrogen sources) on biodegradation of diesel oil were evaluated. optimal dieseloil biodegradation by a. baumanii occurred at initial ph 7, 35°c and initial hydrocarbon concentration at 4%. thebiodegradation products under optimal cultural conditions were analyzed by gc-ms. the present study suggeststhat a. baumannii can be used for effective degradation of diesel oil from industrial effluents contaminated withdiesel oil.