Removal of Zn(II) from electroplating effluent using yeast biofilm formed on gravels: batch and column studies

Background: present study deals with the removal of zn(ii) ions from effluent using yeast biofilm formed ongravels.methods: the biofilm forming ability of candida rugosa and cryptococcus laurentii was evaluated using xtt (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2h-tetrazolium-5-carboxanilide) reduction assay and monitored by scanningelectron microscopy (sem), and confocal laser scanning microscopy (clsm). copious amount of extracellularpolymeric substances (eps) produced by yeast species was quantified and characterized by fourier transforminfrared spectroscopy (ft-ir).results: yeast biofilm formed on gravels by c. rugosa and c. laurentii showed 88% and 74.2% removal of zn(ii) ionsrespectively in batch mode. in column mode, removal of zn(ii) ions from real effluent was found to be 95.29% by c.rugosa biofilm formed on gravels.conclusion: the results of the present study showed that there is a scope to develop a cost effective method forthe efficient removal of zn(ii) from effluent using gravels coated with yeast biofilm.