Bioremediation of p-Nitrophenol by Pseudomonas putida 1274 strain

Background: p-nitrophenol (pnp) occurs as contaminants of industrial effluents and it is the most importantenvironmental pollutant and causes significant health and environmental risks, because it is toxic to many livingorganisms. nevertheless, the information regarding pnp degradation pathways and their enzymes remain limited.objective: to evaluate the efficacy of the pseudomonas putida 1274 for removal of pnp.methods: p. putida mtcc 1274 was obtained from mtcc chandigarh, india and cultured in the minimal mediumin the presence of pnp. pnp degradation efficiency was compared under different ph and temperature ranges. thedegraded product was isolated and analyzed with different chromatographic and spectroscopic techniques.results: p. putida 1274 shows good growth and pnp degradation at 37°c in neutral ph. acidic and alkali phretarded the growth of p. putida as well as the pnp degradation. on the basis of specialized techniques,hydroquinone was identified as major degraded product. the pathway was identified for the biodegradation ofpnp. it involved initial removal of the nitrate group and formation of hydroquinone as one of the intermediates.conclusion: our results suggested that p. putida 1274 strain would be a suitable aspirant for bioremediation ofnitro-aromatic compounds contaminated sites in the environment.