pre-concentration of penicillamine using fe3o4nps and analysis of it in drug formulations through spectroscopic method

First, an effective pre-concentration of penicillamine was done via fe3o4nps. then, a simple, rapid and sensitive spectrophotometric method is described for the determination of penicillamine by 2, 6-dichlorophenolindophenol (2, 6-dcpip), as the chromogenic agent in bulk drug and formulations. it produces a bluish green coloured compound with maximum absorbance 610 nm. beer’s law was obeyed in the concentration range 0.005-0.100 ppm with molar absorptivity 1.97 × 103 l/mol/cm and rsd 0.3-0.82%. statistical comparison of the results with those from the reference method reveals excellent agreement and accuracy and precision was ±0.481 to ±0.33%. this method is also applied on the whole blood and serum samples collected from the confirmed patients. the reaction of penicillamine with 2, 6-dcpip was confirmed by ftir and 1h nmr. the main advantage of 2, 6-dcpip was its stability as a reagent solution, resulting in reliable and reproducible results.