تغییرات صفات فیزیولوژیک و فیتوشیمیایی گیاه شنبلیله (trigonella foenum-groecum) در واکنش به تلقیح با گونه‌های مختلف قارچ مایکورایزا

به‌منظور بررسی و مقایسه تاثیر ده گونه قارچ مایکورایزا متعلق به هفت جنس مختلف (racocetra،وrhizophagus،وclaroideoglomus،وfunneliformis،وdiversispora،وacaulospora و gigaspora) بر برخی صفات رشدی و بیوشیمیایی گیاه شنبلیله، پژوهشی گلدانی بر پایه طرح کاملاً تصادفی در گلخانه پژوهشی دانشگاه فردوسی مشهد انجام شد. در پژوهش حاضر تغییرات زیست‌توده، تعداد و طول غلاف، وزن هزار دانه، رنگیزه‌های فتوسنتزی، ترکیبات فنولی، کربوهیدرات‌های محلول، پروتئین کل، ظرفیت آنتی‌اکسیدانی در گیاه شنبلیله در واکنش به تلقیح مایکورایزایی مورد بررسی قرار گرفت. نتایج تجزیه واریانس داده‌ها نشان داد که اثر قارچ‌های مایکورایزا بر کلیه صفات مورد بررسی در سطح احتمال یک درصد معنی‌دار بود. براساس یافته‌های بدست آمده، واکنش گیاه شنبلیله به تلقیح با گونه‌های مختلف قارچ مایکوریزا متفاوت بود. تلقیح با گونه‌های مناسب قارچ مایکورایزا به‌طور کارآمد از طریق بهبود شرایط رشدی گیاه و صفات بیوشیمیایی منجر به افزایش و بهبود صفات رشدی از جمله زیست‌توده تازه و خشک کل گیاه شنبلیله شد. در پژوهش حاضر همه گونه‌های مایکوریزا به‌ویژه r. castanea،وa. langula،وr. intraradicese و g. margarita میزان کلروفیل و فعالیت‌های آنزیم‌های آنتی‌اکسیدانی گیاه شنبلیله را نسبت به شاهد افزایش دادند که همراه با افزایش رشد گیاه از نظر زیست‌توده تازه و خشک در این تیمارها بود. بر اساس نتایج این پژوهش تلقیح گیاه شنبلیله با قارچ‌های c. claroideum،وr. castanea،وa. langula،وg. margarita،وr. fasciculatus و f. caledonium می‌تواند به‌عنوان جایگزین مناسب کودهای شیمیایی برای افزایش عملکرد این گیاه پیشنهاد شود.

the changes of physiological and phytochemical traits of fenugreek (trigonella foenum-groecum) in response to inoculation with different species of mycorrhizal fungi

to investigate the effect of ten species of mycorrhizal fungi belonging to seven different genera (racocetra,rhizophagus, claroideoglomus, funneliformis, diversispora, acaulospora and gigaspora) on some growthand biochemical traits of fenugreek (trigonella foenum-groecum), a pot experiment based on a completelyrandomized design was conducted in the research greenhouse of ferdowsi university of mashhad. thechanges of biomass, number and length of pods, weight of 1000 seeds, photosynthetic pigments, phenoliccompounds, soluble carbohydrates, total protein, and antioxidant capacity in fenugreek plants in response tomycorrhizal inoculation were studied. the results of variance analysis of the data showed that the effect ofmycorrhizal fungi on all studied traits was significant at the probability level of 1%. based on the findings,the response of fenugreek plant to inoculation with different species of mycorrhizal fungi was variable.inoculation with suitable species of mycorrhizal fungi through improving the growth condition andbiochemical traits of treated plants increased the fenugreek growth based on plant fresh and dry weights. inthe present study, mycorrhizal species, especially r. castanea, a. langula, r. intraradicese, and g. margaritaenhanced the amount of chlorophyll and the activities of antioxidant enzymes of inoculated fenugreek plantcompared to the control, which was accompanied by increasing the plant growth in terms of fresh and dryweights in these treatments. according to the obtained results, inoculation of fenugreek plant with c.claroideum, r. castanea, a. langula, g. margarita, r. fasciculatus, and f. caledonium fungi can besuggested as a suitable alternative for chemical fertilizers to increase the yield of this plant. background and objective: in sustainable agriculture, organic and low input cultivation systems are themost important factors and the application of biofertilizers to reduce chemical fertilizers usage is the mainstep (1). biofertilizers can improve physical, chemical and biological properties of soil and with minimumnegative effects on the environment, could increase soil fertility (2). the application of usefulmicroorganisms such as arbuscular mycorrhizal (am) fungi as an important biofertilizer plays a key role inwater and nutrient supply for plants (3). the purpose of this study was to investigate the effect inoculation with different mycorrhizal species on the growth and biochemical traits of fenugreek. methods: a pot experiment was conducted to investigate the inoculation effect of different species ofmycorrhizal fungi on the growth, physiological and biochemical characteristics of fenugreek (trigonellafoenum-graceum) based on a completely randomized design with three replications in the researchgreenhouse of ferdowsi university of mashhad during 20202021. the treatments included 10 species ofmycorrhizal fungi glomus mosseae, glomus etunicatum, glomus claroideum, glomus caledonium, glomusinteraradices, glomus fasiculatum, acaulospora langula, scutellospora castanea, glomus versiforme,gigaspora margarit and non-inoculation (control). the studied traits included plant fresh and dry weights,number of pods, 1000-seeds weight, photosynthesis pigments, soluble carbohydrate, total phenol, flavonoids,total protein, and antioxidant activity. minitab17 software was used to analyze the data and the meancomparison was performed based on bonferoni test at 1% probability level. results: the results of variance analysis showed that the effect of mycorrhizal fungi on all studied traits wassignificant at the 1% probability level. according to the obtained results, the response of fenugreek toinoculation depended on the mycorrhizal fungi species. among the studied mycorrhizal species, the s.castanea had the greatest effect on improving the plant biomass and photosynthesis pigments. the highestpod number and 1000-seeds weight were obtained in