use of response surface methodology analysis for xanthan biopolymer production by xanthomonas campestris: focus on agitation rate, carbon source, and temperature

The current study is an attempt to contribute for efficient and costeffective substrates for xanthan gum production. in this context, the sugar cane molasses wastes can be used as a cheap substrate for xanthan gum production. xanthan biopolymer production by a novel xanthomonas campestris strain ibrcm 10644 was optimized with statistical approaches. based on the results of response surface methodology (rsm) with central composite design (ccd) technique, a secondorder polynomial model was developed and evaluated the effects of variables on the maximum xanthan production. agitation rate (x1: 200500 rpm), sugar cane molasses concentration (x2: 3090 g/l) and operation temperature (x3: 2535 °c) were the factors investigated. the optimal conditions for maximum yield of xanthan production were derived from agitation rate 500 rpm, carbon source concentration 65 g/l and operation temperature 30°c. under these conditions, xanthan and biomass production were found to be 16.03 g/l and 1.37 g/l, respectively. our results signify that the sugar cane molasses can be used as a cheap substrate for xanthan biopolymer production.