Lipoxygenase-1 Leaching from Soybean Flour Employing Stirred Tank Contactor

The present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. the effect of operating parameters, impellers speed, operational period, temperature, ph and scale-up were considered.the acetic acid ph: 5.2 increased the leaching of lipoxygenase- 1.the sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has been conducted. the effect of agitator speed on the geometrically- scaled- up reactor has shown that similar amount of enzyme is leached at lower speed. the result is in para with the conventional system. the importance of operational period from 5 to 50 min on the enzyme leaching was evaluated and higher enzyme leaching obtained within 10 min. of operation. the sigmaplot-6 for statistical verification and developing a correlation for the enzyme leaching was used.