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   Separation of Biojenic Amines Using Dansyl Cloride Derivatization and Mixed Micellar Liquid Chromatography  
   
نویسنده Hadjmohammadi Mohammad Reza ,Jafari Soghra
منبع iranian journal of chemistry and chemical engineering - 2006 - دوره : 25 - شماره : 1 - صفحه:19 -23
چکیده    Separation of some biogenic amines via rp-hplc using mixed micellar mobile phase was investigated. the compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (sds and brij-35) and the ph of the mobile phase. the number of theoretical plates (n), asymmetry factor (b/a) and selectivity factor (a) considered as criteria for finding the appropriate conditions for separation. appropriate condition, were: 40 °c, 40 m m sds, 0.5 mm brij-35 and 10% 1-propanol at ph=5.
کلیدواژه Biogenic amines ,HPLC ,Micellar liquid chromatography ,Mixed micelles
آدرس university of mazandaran, Department of Chemistry, ایران
پست الکترونیکی m.hadjmr@ umz.ac.ir
 
     
   
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