Bisubstrate Kinetic Model for Enzymatic Decolorization of Reactive Black 5 by Coprinus cinereus Peroxidase

In this study, decolorization of the diazo dye, reactive black 5 (rb5) in a coprinus cinereus peroxidase-catalyzed reaction has been investigated. a bisubstate kinetic model for the reaction rate based on the ping-pong mechanism was assumed for the enzymatic decolorization. experiments were conducted at different rb5 and hydrogen peroxide concentrations in a batch manner to estimate the intrinsic kinetic parameters. these parameters were used for the modeling of decolorization in a continuous reactor that was compared with experimental results. an acceptable agreement was observed between the model and experimental data.