preparation and evaluation of curcumin nano emulsion to inhibit tc-1 cell growth

Curcumin (cur), a substance originating from curcuma longa, has been comprehensively examined for its anticancer properties. nonetheless, its clinical application is restricted by its inadequate solubility, bioavailability, and stability. tc-1 cells have been impressive in understanding hpv biology and developing therapeutic approaches for hpv infection and related cancers, like cervical cancer, offering a close mimicry of hpv-induced carcinogenesis. this study’s primary goal is to formulate and optimize curcumin nanoemulsion (cur-ne) to address these challenges and, secondarily, evaluate their impact on tc-1 cell growth. the characterization of the nanoemulsions was performed by dynamic light scattering (dls), transmission electron microscopy (tem), and high-performance liquid chromatography (hplc). the results revealed an average particle size of 52.5 nm, a zeta potential of -13.1 mv, and a drug content of 96.3%. the dialysis diffusion technique demonstrated that the release of cur from cur-ne is slower and more sustained than free cur. according to an mtt assay, cur-ne with an ic50 35 μg/ml exhibited an increased inhibitory effect of cur on tc-1 cancer cells while showing no inhibitory effects on mc3t3 normal cells at concentrations up to 100 μg/ml. in summary, this study highlights the potential of nanoemulsions as effective carriers for cur, and their safety is demonstrated in both cancer and normal cells. in addition, cur-ne showed significant inhibitory activity against tc-1 cancer cells, suggesting its promising ability in treating hpv-related cancers, especially cervical cancer. further studies are warranted to evaluate the long-term safety of this nanoemulsion for clinical trials and its efficacy against other cancer cell lines.