dodonaea viscosa; enzyme inhibitory potential towards obesity/diabetes management and uhplc-qtof-ms/ms based metabolite profiling

Obesity and diabetes mellitus are generalized under diabesity, a concerning epidemic condition that affects mankind diversely. the medicinal flora has been used as a natural remedy for controlling metabolic disorders like diabesity. dodonaea viscosat (from the potohar region of pakistan) has been evaluated as a potential source for the treatment of obesity/diabetes. different solvent compositions (dvl@100%pee, dvl@80%hee, dvl@60%hee, dvl@40%hee, dvl@20%hee & dvl@pae) were investigated for anti-oxidant, anti-obesity and antidiabetic activities. among all compositions, dvl@60% hee ethanolic leaves extract showed highest tfc (89 ± 1.94 mgre/g de), tpc (144 ± 1.37 mggae/g de), dpph scavenging potential (ic50 = 33.75 ± 0.86 μg/ml), tap (131.59 ±1.30 mg/g aae), pancreatic lipase inhibition (70.82 ± 0.94%) and α-glycosidase inhibition (ic50 = 43.52 ± 0.80 µg/ml). numerous active metabolites have been identified by the highly sensitive uhplc-qtof-ms/ms analytical technique including shikimic acid, cryptomerin b, menisdaurin, 4-o-caffeoylquinic acid, chlorogenic acid, cis-caffeic acid, quinic acid, 4-hydroxymethyl-2-furfural, epicatechin, cnidimol f, 3-hydroxynaringenin, asaricin, cis-anethole, e-p-coumatic acid, methyl 3-o-feruloylquinate, lappaol d, isolariciresinol, and urolignoside, etc that might be responsible for the substantial anti-obesity/antidiabetic attributes of d. viscosa leaves extracts.