optimizing parameters for bio-hydrogen production from mixed culture and food wastewater

In this research, rice boiling wastewater (rbw) has been used as inexpensive food wastewater for bio-hydrogen production by dark fermentation. the effective parameters such as temperature, ph, time reaction, and substrate concentration have been optimized. the 2^k-1 factorial design method and response surface methodology were used to optimize the best reaction conditions. according to the results, under optimal conditions, ph (6.240), temperature (35.090°c), sugar concentration (5.400 g/l), and fermentation time (36 h), the highest hydrogen production in the proposed theoretical model was obtained equal to 3.30 mol h2/mol glucose. the proposed theoretical model demonstrated a good agreement with the obtained experimental data.