synthesis of 6-(phenanthrene-3-yl)-2,3-dihydro-1,2,4,-triazine-3-thione as a new reagent for the non-extractive spectrophotometric determination of cu(ii)

A simple, sensitive, and non-extractive spectrophotometric method was developed for the determination of copper (ii) using a newly synthesized chromogenic reagent, 6-(phenanthrene-3- yl)-1,2,4,-triazine-3-thione(phdtt), in tween 80 neutral surfactant. to synthesize the reagent (phddt), amyl nitrite was added to 3-acethylphenanthrene and refluxed for 26 hours to produce 2-oxo-2-(phenanthren-3-yl) acetaldehyde oxime (i). the reaction of compound (i) with thiosemicarbazide yielded the reagent (phddt). copper (ii) forms a red colored complex with 6-(phenanthrene-3-yl)-1,2,4,-triazine-3-tion which shows maximum absorbance at 460 nm in the ph range 9.0-11.0. beer’s law was obeyed over the concentration range of 1.4-10 μg/ml cu^2+ with r^2 = 0.999. the limit of detection was 0.48 μg/ml of copper (ii). the within-day and between-day precision values were in the range of 0.38-3.3%. the method was tested by analyzing the amalgam samples. the results were in good agreement with the atomic absorption.