kinetic study of ethyl hexanoate synthesis using surface coated lipase from candida rugosa

Kinetics of lipase-catalyzed esterification of hexanoic acid and ethyl alcohol using the solventfree system, surface coated lipase from candida rugosa, had been studied. the effect of various parameters such as reaction time, reaction temperature, reaction kinetics, water removal and feasibility of solventfree system had been focused. candida rugosa lipase was more effective than other lipases when ethyl hexanoate was synthesized in nhexane. the highest esterification yield after 72 h (93 %) was achieved at a ph of 5.2 and the esterification yield was reduced to 73% at ph 4.0. the values of the apparent kinetic parameters were computed as vmax= 0.146 μmol/min/mg enzyme; km, acid = 0.296 m; km, alcohol = 0.1388 m; ki, acid = 0.40 m; and ki, alcohol = 0.309 m. the reaction rate could be described in terms of the michaelis–menten equation with a ping-pong bibi mechanism and competitive inhibition by both the substrates.