Decolorization of Maxilon-Red by Kissiris Immobilized Phanerochaete Chrysosporium in a Trickle-BedBioreactor-Involvement of Ligninolytic Enzymes

The decolorization of maxilon-red dye by kissiris immobilized phanerochaetechrysosporium in the trickle-bed reactor (tbr) using the basal nitrogen-limited growth mediumwas studied. the influence of the superficial liquid velocity (slv) on the decolorizing ability of thefungus was examined at four slvs (cm sec-1): 0.05, 0.075, 0.1, and 0.15. maximum level of thedecolorizing activity was about 94 % by day 4-5 of the process when the tbr operated atslv=0.075 to 0.1 cm sec-1. at these slvs, the maximum activities of the lignin peroxidase (lip)and the manganese peroxidase (mnp) were (ul-1): 87 and 207, respectively. in a successivedecolorization of the tested dye in a continuous dye addition in the tbr when glucose leveldecreased to 2 gl-1, significant reduction in the characteristics of the interest were observed. whileby controlling the glucose concentration, these properties were improved considerably: thedecolorizing ability of the kissiris immobilized mycelia was more than 85 % after nearly 20 days ofthe process while the maximum activities of the lip and the mnp were (ul-1): 320 and 151,respectively. considerable levels of the enzyme activities were detected by day 19 and after 11 timesof the dye additions.