Isolation and adaptation of diatoms in a new formulated enriched medium

This study describes the adaptations of diatoms,cylindrotheca fusiformis and other marine diatoms,in a new formulated enriched medium tris-phosphate seawater (tp-sw). the medium was designed to maintain long-term cultures of wide-range marine diatoms in laboratory that produces high biomass of cultures. the diatoms were adapted and cultivated in the medium for 15 days and the number of cells was recorded daily. it was found that the number of cells declined after two weeks indicating death phase of the cells. this indicates that the tp-sw medium has supported the growth of diatoms during the period and can be used to cultivate diatoms in vitro. studies on the tp-sw medium must be done to obtain optimal medium that can provide not only a conducive environment for the survival of diatoms but also high biomass production. © 2017 universiti putra malaysia press.