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transient expression of coat protein of foot and mouth disease virus (fmdv) in alfalfa (medicago sativa) by agroinfiltration
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نویسنده
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habibi-pirkoohi maziar ,malekzadeh-shafaroudi saeid ,marashi hasan ,zibaee saeid ,mohkami afsaneh ,nejatizadeh saba
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منبع
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journal of cell and molecular research - 2016 - دوره : 8 - شماره : 2 - صفحه:83 -89
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چکیده
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An agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain reaction (pcr), real time pcr, protein dot blot and elisa. moreover, gene expression in the transformed leaves was quantified by elisa method. the results obtained in this investigation indicated high level of gene expression in alfalfa leaves, showing that transient gene expression can be applied as an effective and time-saving procedure for the production of recombinant proteins. the procedures for transformation, detection of recombinant protein and its application for molecular experiments are described in the study.
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کلیدواژه
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agroinfiltration ,fmdv ,recombinant vaccine ,alfalfa ,vp1
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آدرس
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ferdowsi university of mashhad, department of biotechnology and plant breeding, ایران, ferdowsi university of mashhad, department of biotechnology and plant breeding, ایران, ferdowsi university of mashhad, department of biotechnology and plant breeding, ایران, ministry of agriculture, razi vaccine and serum research institute, ایران, shahid bahonar university of kerman, research and technology institute of plant production, ایران, ferdowsi university of mashhad, department of biotechnology and plant breeding, ایران
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پست الکترونیکی
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s.siklamen@yahoo.com
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Authors
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