Neuronal Differentiation of Mouse Amnion Membrane Derived Stem Cells in Response To Neonatal Brain Medium

Amniotic membrane derived stem cells have considerable advantages for being used in regenerative medicine and their anti-inflammatory effects, growth factor secretion and differentiation potential, have made them suitable candidates for nervous system stem cell therapy. the developing and neonatal brain contains a spectrum of growth factors to direct development of endogenous and donor cells. using an in vitro model, we have investigated the plasticity and potential of mouse amnion membrane stem cells to be differentiated into neural cells in response to neonatal mice brain extracted medium. mouse amniotic membrane stem cells were isolated from embryonic membrane and confirmed by flow cytometric analysis for their surface markers. harvested stem cells were cultured in neonatal brain derived medium for 21 days and neural differentiation of cells was explored using immunohistochemistry and flowcytometry analysis. isolated amnion membrane stem cells showed high rate of viability and proliferation and also expressed stem cell markers such cd105 and cd90. amnion stem cells presented neural characters such as morphological changes and development of axon like appendixes as well as nestin and map-2 expression when came in contact with brain tissue. in conclusion, the current investigation showed that amnion membrane derived stem cells are potent cells for responding to environmental signals promoting them to neural fate and could be used in regenerative medicine of neurodegenerative disorders.