A Comparative Investigation on Efficiency of Bacteriophage Lambda and M13 Based Vectors For Delivering and Expression of Transgene in Eukaryote Cells

Gene delivery might be affected by several tribulations based on carrier/vector applied. bacteriophages lambda andm13 have different genome conformations; linear double-stranded and circular single-stranded respectively.therefore, it might be expected that these two common classes of gene delivery vehicles will have differentcapacity for gene delivery and expression in eukaryote cells. to address the possible effects of linear doublestrandedand circular single-stranded genome conformations of bacteriophages lambda and m13 on the transgeneexpression, the transfection efficacy of two vectors based on lambda and m13 were compared in ags cell line. thegfp encoding sequence was inserted into the lambda zap-cmv xr vector which resulted in ?-zap-cmv-gfpconstruct. the construct was then in vitro packaged using gigapack® iii gold packaging extract and ?-gfp phageparticles were obtained. the ?-gfp phage particles were then used for in vivo excisioning which resulted in m13-cmv-script-gfp construct. 1011 copy of ?-zap-cmv-gfp or m13-cmv-script-gfp constructs were transfectedinto ags cells using lipofectamine 2000. transfection efficiencies were analyzed by facs. results showed thatlinear double-stranded ?-zap-cmv-gfp was efficient than single-stranded form of m13-cmv-script-gfp whileits double-stranded form was efficient than the linear double-stranded ?-zap-cmv-gfp construct for transgenedelivery and expression. moreover the gfp signals resulted from transfections by single-stranded form of m13-cmv-script-gfp construct faded more quickly in comparison to others. these findings highlight that genomeconformation of gene carriers might be an important factor when seeking for an appropriate gene carrier/vehicle.