|
|
Allelic polymorphism of K-casein, B-Lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle
|
|
|
|
|
نویسنده
|
Mohammadi Yahya ,Aslaminejad Ali Asghar ,Nassiri MohammadReza ,Koshkoieh Ali Esmailizadeh
|
منبع
|
journal of cell and molecular research - 2013 - دوره : 5 - شماره : 2 - صفحه:75 -80
|
چکیده
|
The purpose of this study was to investigate the polymorphism of k-casein (k-cn), b-lactoglobulin (b-lg) and leptin (lp) genes in iranian holstein cattle by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) technique. dna was extracted from blood samples of 139 cows using a modified phenol chloroform method. association between k-cn, b-lg and lp genes’ polymorphism with milk production traits were investigated using mixed procedure of sas software. the frequencies of aa, ab and bb genotypes for k-cn (0.72, 0.18 and 0.10), b-lg (0.43, 0.28 and 0.29) and lp (0.24, 0.63 and 0.13) were also calculated. statistical results revealed a significant association between aa and bb genotypes of the k-cn gene with milk production and milk protein percentage, respectively. also, bb genotype of the b-lg gene and aa genotype of the lp gene showed a significant association with protein percentage and milk production (p < 0.05), respectively. therefore, it is feasible to improve milk production traits in these herds using the studied genes.
|
کلیدواژه
|
Holstein cattle ,K-casein ,B-Lactoglobulin ,leptin gene ,PCR-RFLP ,milk production traits
|
آدرس
|
ferdowsi university of mashhad, ایران, ferdowsi university of mashhad, ایران, ferdowsi university of mashhad, ایران, shahid bahonar university of kerman, ایران
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|