|
|
Association of growth trait and Leptin gene polymorphism in Kermani sheep
|
|
|
|
|
نویسنده
|
Shojaei Moslem ,Mohammad Abadi Mohammadreza ,Asadi Fozi Masood ,Dayani Omid ,Khezri Amin ,Akhondi Masoumeh
|
منبع
|
journal of cell and molecular research - 2010 - دوره : 2 - شماره : 2 - صفحه:67 -73
|
چکیده
|
Identification of genes affecting energy balance, milk yield and feed intake is an interesting area ofresearches in animal breeding. leptin gene polymorphism is associated with key economic affair. considering rich resources for animals, in our country, accomplishing a few assays to identify a gene that controls her traits with molecular genetics, and identifying the candidate genes in sheep breeds using dna test can greatly help to her breeding progress. for analyzing leptin gene polymorphism and its association with growth traits in kermani sheep, blood samples of ]20 sheep of both genes rearing at breeding centre of shahre babak were taken. in addition growth traits were measured. pcr was performed to amplify 275 bp fragments of exon 3 from leptin gene. then single strand conformation polymorphism (sscp) of pcr product was performed and leptin band patterns (genotypes) were obtained using acrylamid gel and silver staining. for leptin gene 10 genotypes including ala, c/c, alb, a/c, albic, albie, a/b/f, a/c/f, aibidie and aiblelf were obtained. the results of this study showed that the growth traits are significantly affected by the genotypes. accordingly, albie, a/c, a/b/cif and aib/c/f genotypes had higher body weight at 3, 6, 9 and 12 months of ages respectively. the animals with alb, alb, a/bif and aibid/e genotypes had the smallest body weight at 3, 6, 9 and 12 months of ages respectively. it is suggested that polymorphism in leptin gene loci can be used as a selective criterion to improve growth traits in kennani sheep.
|
کلیدواژه
|
Growth traits ,Kermani sheep ,Leptin ,PCR-SSCP ,Polymorphism
|
آدرس
|
shahid bahonar university of kerman, Faculty of Agriculture, Department of Animal Science, ایران, shahid bahonar university of kerman, Faculty of Agriculture, Department of Animal Science, ایران, shahid bahonar university of kerman, Faculty of Agriculture, Department of Animal Science, ایران, shahid bahonar university of kerman, Faculty of Agriculture, Department of Animal Science, ایران, shahid bahonar university of kerman, Faculty of Agriculture, Department of Animal Science, ایران, shahid bahonar university of kerman, Faculty of Agriculture, Department of Animal Science, ایران
|
پست الکترونیکی
|
mmohammadabadi@yahoo.com
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|