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   systems biology analysis of the key genes of surfactin production in bacillus subtilis mj01 (isolated from soil contaminated oil in south of iran), spizizenii, and 168 isolates  
نویسنده deihimi tahereh ,ebrahimie esmaeil ,niazi ali ,ebrahimi mansour ,ayatollahi shahab ,tahmasebi ahmad ,rahimi touraj ,jahanbani veshareh moein
منبع journal of cell and molecular research - 2017 - دوره : 9 - شماره : 2 - صفحه:84 -97
چکیده    applying microorganism in oil recovery has attracted attentions recently. surfactin produced by bacillus subtilis is widely used industrially in a range of industrial applications in pharmecutical and environmental sectors. little information about molecular mechanism of suffactin compound is available. in this study, we performed promoter and network analysis of surfactin production genes in bacillus subtilis subsp. mj01 (isolated from oil contaminated soil in south of iran), spizizenii and 168. our analysis revealed that comq and comx are the genes with sequence alterations among these three strains of bacillus subtilis and are involved in surfactin production. promoter analysis indicated that lrp, argr, rpod, purr and ihf are overrepresented and have the highest number of transcription factor binding sites (tfbs) on the key surfactin production genes in all 3 strains. also the pattern of tfbs among these three strains was completely different. interestingly, there is distinct difference between 168, spizizenii and mj01 in their frequency of tfs that activate genes involve in surfactin production. attribute weighting algorithms and decision tree analysis revealed ihf, rpod and flhcd as the most important tf among surfactin production. network analysis identified two significant network modules. the first one consists of key genes involved in surfactin production and the second module includes key tfs, involved in regulation of surfactin production. our findings enhance understanding the molecular mechanism of surfactin production through systems biology analysis.
کلیدواژه surfactin production gene ,transcription factor (tf) ,promoter analysis ,network analysis
آدرس shiraz university, institute of biotechnology, ایران, shiraz university, institute of biotechnology, ایران. university of adelaide, adelaide medical school, faculty of health and medical sciences, australia, shiraz university, institute of biotechnology, ایران, university of qom, department of biology, ایران, sharif university of technology, school of chemical & petroleum engineering, ایران, shiraz university, college of agriculture, department of crop production & plant breeding, ایران, shiraz university, institute of biotechnology, ایران, danish hydrocarbon research and technology center, denmark
پست الکترونیکی moein@dtu.dk

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