comparison of genetic diversity and growth traits among fangzheng silver crucian carp (carassius auratus gibelio) gynogenetic clones

The silver crucian carp (carassius auratus gibelio), a gynogenetic teleost, is a promising model for the study of evolutionary genetics in vertebrates. we identified ten gynogenetic clones (fz-i~fz-x) from triploid silver crucian carp, collected from fangzheng county in heilongjiang province, china, using microsatellite markers. the genetic diversity of these gynogenetic clones was analyzed using 52 microsatellite markers. a total of 413 alleles were detected and the length of fragments ranged from 96 to 340 bp. the number of alleles per locus varied from 2~19 (mean=7.9423). the observed heterozygosity at polymorphic loci ranged from 0.10~1.00 (mean=0.80). the average allele count per gynogenetic clone ranged from 1.9423~2.1923. the ratio of the polymorphic locus was from 71.15% (vii) to 84.61% (ix) per clone. the number of genotypes ranged from 2~10 per locus. ten genotypes were observed by analyzing each of 14 microsatellites. as a result, each gynogenetic clone could be accurately identified. in addition, the growth traits, including body weight, length, and height, among five gynogenetic clones were compared. there was a significant difference among gynogenetic clones. clone fz-v exhibited the best growth traits, with the largest body weight (53.17±5.24 g), length (11.38±0.37 cm) and height (4.69±0.18 cm). our results provide basic data for the identification of silver crucian carp gynogenetic clones and can be used as a guide genetic breeding programs.