Effect of different pH and salinity levels on the viability of Penaeus monodon baculovirus (MBV) in Penaeus smisulcatus.

The virucidal effect of ph and salinity on penaeus monodon baculovirus(mby) was investigated by water borne inoculated methods exposing pl12 penaeus semisulcatus for 10 hours and observing for 10 days at temperature 2830° c. for this purpose, one thousand virus free pl12 of p. semisulcatus were divided into five groups and each group placed in a 10 l plastic basin containing chlorine-treated sea water at 35ppt. the salinity of basins water after 12 hours changed to 5, 10, 20, 30 and 40ppt using synthetic sea salt. to study the effect of ph, solution with various ph of 3, 5, 7, 8, 9 and 12 were prepared using in naoh and in hcl to na2hpo. solution. for transmission of mby, an aliquot ofapproximately 10 frozen infected larvae were used for each experiment by water born inoculated method. ten hours after inoculation, the shrimp were removed from jar and introduced into another jar with the same salinity or ph. during 10 days experiment, the rate of infection (roi), severity of infection (sol) and accumulative mortality were varied among the mby-infected. penaeus mondon baculovirus was able to infect penaeus semisulcatus at salinity ranging from 5-40ppt whereas at ph 3and 12, mby was completely inactivated. mby was not able to infect larvae at ph 5,7,8 and 9.