Some hematological and biochemical changes in blood serum of Beluga (Huso huso) after chronic exposure to diazinonSome hematological and biochemical changes in blood serum of Beluga (Huso huso) after chronic exposure to diazinon

Hematological and biochemical changes were studied in beluga (husohuso) weighing 450(plus-minus)50g in the presence of p,l-3, d-glucan as a singleintraperitoneal injection (o.3mg/kg body weight) once, and thereafter a chronicexposure to organophosphate, diazinon at concentration of 1.5 mg/l as a continuousbath for 63 days at 22(plus-minus) 1°c. results of erythrocyte profile in fish treated with glucan,but without diazinon, generally showed significant higher values of rbc (p(less than)0.05)compare to fish treated with glucan plus diazinon bath, while there was nosignificant difference (p(greater than)0.05) in the values. of hct, hb, mch and mchc betweenthe two groups. also, fish treated with glucan, but without diazinon bath, showedsignificant higher values of rbc than that untreated fish up to 3 weeks posttreatment(p(less than00.05), while no significant differences was found in other erythrocyticindices between these two groups (p(greater than)0.05). furthermore, fish treated with diazinonshowed higher levels of alkaline phosphatase (alp), alanine aminotransferase(alt), lactate dehydrogenase (ldh) than other three groups for 14-28 days posttreatmentand then they decreased to the lower level for the rest of experiment. also,fish treated with glucan-diazinon showed insignificant higher levels of theseenzymes up to 21 days post-exposure compare to fish treated with glucan butwithout diazinon bath (p(greater than)0.05). however, such levels reduced to a lower level in thisgroup for the rest of experiment (p(greater than)0.05). also, fish treated with glucan-diazinonshowed generally lower and higher levels of total protein and glucose concentrationsin blood plasma, respectively compared to fish treated with glucan but withoutdiazinon bath (p(less than(0.05).