Analysis of Saline Activator Solution Effects on Sperm Quality Indices of Barbus Sharpeyi By Image J Software

Regarding low fertilization rate of barbus sharpeyi, one of the main barbus species in south of iran, saline activator solutions (sas 1 and 2) were used for increasing of sperm activation and its sperm quality indices were assessed by using image j software as a fish sperm adapted tools. sas1 (45mm nacl, 5mm kcl, 30mm tris, 188±2.3 mosmol/kg) and sas2 (50mm nacl, 30mm kcl, 30mm tris, 189±1.7 mosmol/kg) were added to a mixture of 12 male semen comparing with distilled and tap water and their effects on vcl, vsl, alh, linearity, %motility, spermatozoa path, % a, b and c spermatozoa type, fertilization, hatching and deformity rate were analyzed in triplicate. results showed that sas2 had the highest vcl (21.37±0.36 µm/s), vsl (11.6±0.3 µm/s) and motility percentage (85.75±1.56 %) among treatments. the highest a type spermatozoa percentage was recorded for sas1 (47.16±3.15 %) and sas2 (43.89±4.54%). the lowest c type spermatozoa percentage was recorded using sas2 (14.24±1.56 %). also, the highest fertilization rate was achieved from sas2 (79.5±0.37%) and sas1 (77.74±4%) respectively. furthermore the lowest larval deformity rate (13.77±1.03%) was shown due to the use of sas2. final conclusion confirmed that sas1 and sas2 have positive and significant effects (p < 0.05) on extending the time length of sperms movement, vcl and vsl of b. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of iran.