karyoevolutive consideration of two sea fish species via the newest short-term culture method

This study successfully determined the chromosome numbers and karyotypes of two marine fish species from separate families using the short-term culture method (pb-max™ application). the culture incubation periods varied for each species. kidney tissues from the fish samples were treated with pb-max™ for either 2.5 or 4 h. results showed that diploid chromosome numbers, karyotypes, and arm numbers differ by species: red mullet, mullus barbatus, has 2n – 44, with 3 pairs of metacentric, 7 pairs of submetacentric, 7 pairs of subtelocentric, and 5 pairs of acrocentric chromosomes (nf=64). round goby, neogobius melanostomus, has 2n – 46, consisting of 46 pairs of acrocentric chromosomes (nf=46). c– and nor–positive results were achieved through constitutive heterochromatin banding and silver nitrate staining for both species. the primary objective of this research is to demonstrate the applicability of short-term culture in producing successful cytogenetic results in marine fish. this study will support future research and facilitate chromosome studies on marine fish, making the process quick and practical.