Research Article:Effects of Dietary Selenium and Zinc Nanoparticles on Growth Performance, Immune Responses, and Antioxidant Enzymes Activities O White Leg Shrimp (Litopenaeus Vannamei)

In the present study selenium nanoparticles (se-nps) and zinc nanoparticles (zn-nps) included in the diets of white shrimp at 0 mg (control), 0.3 mg se-nps/ kg feed (t1), 0.15 mg se-nps+15 mg zn-nps/kg feed (t2) and 30 mg zn-nps/kg feed (t3). after 8 weeks, shrimps fed both se-nps and zn-nps showed higher (p<0.05) final body weigh, weigh gain, and sgr than shrimp fed the control diet. on the other hand, the fcr was decreased by se-nps and zn-nps when compared to the control whereas both se-nps+zn-nps revealed lower fcr than the other groups (p<0.05). shrimp fed se-nps or se-nps +zn-nps revealed higher survival rate than shrimp fed the control and zn-nps while shrimp fed zn-nps showed higher survival rate than shrimp fed the control (p<0.05). the total haemocyte count, large granular cells, semi granular cells, and semi granular cells revealed higher counts in shrimps fed se-nps and zn-nps than the control (p<0.05). shrimp fed se-nps or se-nps+ zn-nps revealed higher catalase (cat), superoxide dismutase (sod), and glutathione peroxidas (gpx) activities than shrimp fed the control and zn-nps while shrimp fed zn-nps showed higher catalase activity than shrimp fed the control (p<0.05). the total protein, lysozyme, and phenoloxidase activities increased in shrimp fed se-nps or/and zn-nps with the highest being in shrimp fed both in shrimp fed se-nps and zn-nps followed by those fed se-nps then zn-nps when compare to the control (p<0.05). in conclusion, the co supplementation of se-nps and zn-nps showed higher performances, antioxidant status, and immune responses in white shrimp than the individual supplementation of se-nps or zn-nps.