extraction and characterization of collagen from the skin and bone of shabout (arabibarbus grypus heckel, 1843)

Acid soluble collagen (asc) from the skin (asc-s) and bone (asc-b) of shabout (arabibarbus grypus heckel, 1843) were isolated and characterized. both asc-s and asc-b from shabout contained glycine as the major amino acid and high amount of proline (pro), hydroxyproline (hyp), alanine and glutamic acid. on the basis of dry weight, yields of asc-s and asc-b were 6.79 and 2.57%, respectively. furthermore, fourier transform infrared spectroscopy (ftir) proved that both collagens were integrated and native. the denaturation temperature of asc-s and asc-b were 31.59 and 32.25°c, respectively. additionally, the results of x-ray diffraction (xrd) proved that the two products retained their helical structures. these collagens had prominent absorptions at 230 nm by uv-vis spectra. additionally, the scanning electron microscope (sem) studies have shown that acs-s and asc-b are porous and exhibited fibrous nature. according to the uv–vis and ftir results, extracted collagens were characterized as type i collagen based on their amino acid profile. in the current study, the total amount of amino acid (pro+hyp) was 19.28% and 19.55% for asc-s and asc-b, respectively. the results of the current study suggested that the collagen isolated from shabout can potentially be an alternative source for use in the food, pharmaceutical and biomedical industries.