carotenoprotein from by-product of banana shrimp (penaeus merguiensis) extracted using protease from viscera of rainbow trout: antiradical and angiotensin i-converting enzyme inhibitory activity

Carotenoprotein as a marine bioactive compound was recovered from banana shrimp byproduct using protease from rainbow trout viscera at various levels (5 and 15 units/g shrimp shell). the yield of carotenoprotein increased with increasing enzyme concentration up to 51.6%, which was consistent with increasing degree of hydrolysis. protein and lipid content of the hydrolysate was 79.6 and 9.4 percent, respectively. the hydrolysate had a high nutritional value with a high amount of essential amino acids (465 mg/g) compared to the control (254 mg/g). the carotenoprotein exhibited a dosedependent increase in antiradical activity measured by two in vitro assays, with an efficiency of 94 and 57 percent for dpph and abts scavenging activities, respectively. protease-extracted carotenoprotein showed higher ace inhibiting effect than the control at different concentrations (p<0.05). the results from this study showed that carotenoprotein from banana shrimp have both radical scavenging activity and antihypertensive properties and a high content of essential amino acids, and could be good source for valueadded nutritive food ingredients.