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the role of m2000 as an anti-inflammatory agent in toll-like receptor 2/microrna-155 pathway
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نویسنده
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pourgholi fatemeh ,hajivalili mahsa ,razavi rasoul ,esmaeili shadi ,baradaran behzad ,movasaghpour ali akbar ,sadreddini sanam ,goodarzynejad hamidreza ,mirshafiey abbas ,yousefi mehdi
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منبع
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avicenna journal of medical biotechnology - 2017 - دوره : 9 - شماره : 1 - صفحه:8 -12
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چکیده
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Background: m2000 is a newly designed and safe non-steroidal anti-inflammatory drug (nsaid). the aim of this study was to assess the effects of m2000 on expression levels of suppressor of cytokine signaling-1 (socs-1) and src homology-2 domaincontaining inositol-5’-phosphatase 1 (ship1) proteins via toll-like receptor (tlr) 2/microrna-155 pathway. methods: hek293 tlr2 cell line and peripheral blood mononuclear cells (pbmcs) were treated by different concentrations of m2000 in mtt assay. rna was extracted by mirneasy mini kit. then, cdna was synthesized and the expression levels of socs1, ship1 and mirna155 were evaluated by quantitative real time pcr. results: our results showed that m2000 significantly increased the expression levels of socs1 and ship-1 in lipopolysachride (lps)-treated and non-treated cells. moreover, m2000 decreased expression level of mir-155 in lps treated pbmcs. conclusion: m2000 can be used as nsaid in lps induced inflammation and decrease inflammatory cytokines production by targeting socs1, ship1 and mir-155 in autoimmune and inflammatory diseases.
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کلیدواژه
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micrornas ,ship1 ,socs1
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آدرس
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tabriz university of medical sciences, hematology and oncology research center, immunology research center, faculty of medicine, department of immunology, ایران, tabriz university of medical sciences, hematology and oncology research center, immunology research center, faculty of medicine, department of immunology, ایران, tarbiat modares university, department of hematology and blood banking, ایران, tarbiat modares university, department of hematology and blood banking, ایران, tabriz university of medical sciences, immunology research center, faculty of medicine, department of immunology, ایران, tabriz university of medical sciences, hematology and oncology research center, faculty of medicine, department of immunology, ایران, tabriz university of medical sciences, immunology research center, ایران, tehran heart center, basic and clinical research department, ایران, tehran university of medical sciences, faculty of public health, department of immunology, ایران, tabriz university of medical sciences, immunology research center, faculty of medicine, department of immunology, ایران
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پست الکترونیکی
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yousefime@tbzmed.ac.ir
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Authors
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