Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes

Background: application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features. methods: in the present study, recombinant hbsag encapsulated into methanobrevibacter smithii (m. smithii) archaeosomes. balb/c mice immunized with this compound and humoral and cytokine secretion pattern of immunized models analyzed. results: frequency of ifn-γ secreting cells in the hbsag-containing archaeosomes group was significantly higher than hbsag and hbsag+c/ifa groups (p≤0.05). igg2a titer in the sera of hbsag-containing archaeosomes group was also significantly higher than this subclass titer in the other groups (p≤ 0.05). conclusion: analysis of induced responses revealed the immunopotentiating characteristics of m. smithii archaeosomes in the induction of t-helper 1 responses according to the dominance of igg2a subtype and ifn-γ secreting splenocytes of immunized mice.