Downregulation of Mmp2 and Bcl-2 in Adipose Derived Stem Cells (Ascs) Following Transfection With Ip-10 Gene

Background: mesenchymal stem cells (mscs) are recently introduced as novel immunological gene carriers for treatment of cancer. it is believed that balance between the expression of angiogenic and anti-angiogenic factors, such as sdf-1 and ip-10, may regulate neovascularization within the tumor. methods: in this study, we compared the expression of important tumor promoting mediators in ip-10-transfected adipose derived stem cells (ascs) to those transfected with sdf-1. ascs were isolated from adipose tissue of a normal subject undergoing cosmetic mamoplasty surgery using collagenase. ascs were transfected with ip-10 or sdf-1 propagated plasmids by electroporation method and lipofectamin 2000. expressions of sdf-1, cxcr4, ip-10, bcl-2, mmp2, il-10, igf-1, and vegf were detected in transfected ascs using quantitative real-time polymerase chain reaction (qrt-pcr). results: results showed that the expressions of sdf-1, cxcr4, bcl-2, mmp2, il-10, igf-1, and vegf were upregulated in sdf-1-transfected ascs. in contrast, bcl-2 and mmp2 transcripts showed 45×10³ and 10 fold lower expression in ascs transfected with ip-10 compared to non-transfected cells. conclusion: anti-angiogenic chemokines such as ip-10 may modulate tumor promoting properties of ascs and would be introduced as novel candidates for tumor immunotherapy; however, further studies are needed to be conducted.