Higher Improvement of Cardiac Function Following Myocardial Infarction usingMenstrual Blood Stromal/Stem Cells (MenSCs) Suspended in Conditioned Medium versusConditioned Medium Alone in Rat Model

Background: to evaluate the efficiency of menstrual blood stromal/stem cells (menscs) administration in myocardial infarction (mi), the effects of menscs and their derived conditioned medium (cm) on cardiac function in mi rat model was assessed. methods: animals were divided into four groups including sham group, mi group, menscs derived cm group (cm group), and menscs suspended in cm (menscs+ cm) group. the injection of different groups was carried out 30 min after ligation of left anterior descending coronary artery into the infarct border zone. results: the results showed a significant reduction in scar size after injection of menscs+cm compared to mi group. ejection fraction and fractional shortening of menscs+cm group were higher than cm and mi group at day 28. administration of menscs+cm led to much more survival of cardiomyocytes, and prevention of metaplastic development. moreover, human mitochondrial transfer from menscs to cardiomyocytes was seen in group treated by menscs+cm. indeed, menscs+cm treatment evoked nuclear factor-κb (nf-κb) down-regulation more than other treatments. conclusion: menscs+cm treatment could significantly ameliorate cardiac function by different mechanisms including inhibition of cartilaginous metaplasia, inhibition of nf- κb and mitochondrial transfer.