Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis using Conditioned Media of Probiotics Bacteria

Background: stem cells are used to treat numerous diseases; however, their lifespan is rather short. factors such as probiotics affect and improve various cell lineage efficacies. the aim of this study was to investigate the effects of probiotics-conditioned media on dental pulp stem cell potentials in osteogenesis. methods: the experiment was initiated by culturing lactobacillus casei and lactobacillus acidophilus probiotics as well as dps-7 cells. bacterial supernatants were separated and concentrated as the conditioned media. the dps-7 cells were treated with various concentrations of the conditioned media. furthermore, mtt assay and alkaline phosphatase activity were used. the mrna expression of three genes (bfgf, egf-β and bmp-2) involved in osteogenesis was analyzed using a real-time polymerase chain reaction. results: the response of dental pulp stem cells to probiotics preconditioning promoted cell proliferation, increased alkaline phosphatase activity and upregulated bfgf and bmp-2 gene expression. increased expression was significant for bmp-2 and moderate for bfgf; however, it was non-significant for egf-β. the use of the two probiotics was the most effective. conclusion: in general, synergism of the combined probiotics preconditioning induces differentiation of dps-7 cells into osteoblasts most effectively.