Comparative Antioxidant and Anti-gout Activities of Citrullus colocynthis loaded Fruit Silver nanoparticles with its Ethanolic extract

Background: the biological synthesis of silver nanoparticles (agnps) using plant materials is a rapidly developing method with several alternative medical applications. this comparative study of ethanolic fruit extract of citrullus colocynthis (c. colocynthis) (efecc) and synthesized silver nanoparticles (cc-agnps) were carried out for antioxidants and anti-gout arthritic activities. methods: the agnps were synthesized using c. colocynthis fruit and its characterization was done by uv-visible spectroscopy, tem, xrd and ft-ir. the 90% ethanol was used for extract preparation. antioxidant activity was analyzed by dpph and the hydrogen peroxide (h2o2) method. in vitro anti-arthritic activity was tested by xanthine oxidase inhibition, protein denaturation and hrbc membrane stabilization assay. results: the synthesized cc-agnps were confirmed by uv-vis spectroscopy and tem images displayed spherical shapes with 10-45 nm size range. furthermore, the functional groups and crystalline structure of cc-agnps were determined by ft-ir and xrd analysis. the biosynthesized cc-agnps exhibited an excellent free radical scavenging ability than efecc. in anti-arthritic activity, the cc-agnps showed effective inhibition of xanthine oxidase production, protein denaturation, and damaged rbc membranes compared to efecc. conclusion: the antioxidant activities and in vitro anti-arthritic assays revealed that cc-agnps are better anti-gout agents than efecc. this research suggested that biosynthesized silver nanoparticles from c. colocynthis fruit are an important target in the field of anti-gout drug discovery.