Designing a Multi-Epitope Antigen for Serodiagnosis of Strongyloides stercoralis Based on L3Nie.01 and IgG Immunoreactive Epitopes

Background: serological diagnosis of strongyloides stercoralis (s. stercoralis) is fre-quently challenging because of cross-reactivity with other parasitic nematodes. there-fore, it is necessary to introduce novel serological tests with high performance to properly diagnose this neglected parasitic infection. the purpose of the current study was to design a multi-epitope construct for the diagnosis of s. stercoralis.methods: for the purpose of this study, first, highly antigenic segments and potential immunodominant epitopes of s. stercoralis were identified from two antigenic proteins, and then all of the selected parts were linked by an appropriate linker. next, the physico-chemical features of the designed construct were analyzed. then, tertiary structures of the construct were built and evaluated to find out the best one. lastly, the amino acid sequence was reverse-translated and optimized for over-expression in escherchia coli (e. coli).results: the bioinformatic evaluation indicated that the designed protein construct could be hydrophilic, thermostable, and acidic and the estimated half-life was more than 10 hr in e. coli.conclusion: according to the results of the study, the designed construct could be used as an efficient antigen in the elisa system for serological diagnosis of human strong-yloidiasis.