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Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells
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نویسنده
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simonian miganoosh ,haji ghaffari mozhan ,salimi ali ,mirzadegan ebrahim ,sadeghi niloufar ,ebrahimnezhad nasim ,fazli ghazaleh ,fatemi ramina ,bayat ali-ahmad ,milani saeideh ,negahdari babak ,rabbani hodjattallah
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منبع
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avicenna journal of medical biotechnology - 2022 - دوره : 14 - شماره : 1 - صفحه:37 -45
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چکیده
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Background: sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells. methods: in this study, the expression of sortilin in 4t1 and mda-mb231 cell lines was evaluated by flow cytometry and immunocytochemistry. apoptosis assay was also applied to evaluate apoptosis induction in 4t1 and mda-mb231 cell lines. results: based on cell surface flow cytometry results, anti-sortilin (2d8-e3) mab could recognize sortilin molecules in 79.2% and 90.3% of 4t1 and mda-mb231 cell-lines, respectively. the immunocytochemistry staining results confirmed sortilin surface expression. apoptosis assay indicated that anti-sortilin mab could induce apoptosis in 4t1 and mda-mb231 cell lines. conclusion: our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies.
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کلیدواژه
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Breast cancer ,Flow cytometry ,Monoclonal antibody ,Sortilin
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آدرس
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tehran university of medical sciences, school of advanced technologies in medicine, department of medical biotechnology, Iran, tehran university of medical sciences, school of advanced technologies in medicine, department of medical biotechnology, Iran, academic center for education, culture and research (acecr ), monoclonal antibody research center, avicenna research institute, Iran, academic center for education, culture and research (acecr), nanobiotechnology research center, avicenna research institute, Iran, academic center for education, culture and research (acecr), monoclonal antibody research center, avicenna research institute, Iran, academic center for education, culture and research (acecr ), monoclonal antibody research center, avicenna research institute, Iran, academic center for education, culture and research (acecr), monoclonal antibody research center, avicenna research institute, Iran, academic center for education, culture and research (acecr), monoclonal antibody research center, avicenna research institute, Iran, academic center for education, culture and research (acecr ), monoclonal antibody research center, avicenna research institute, Iran, high institute for research and education in transfusion medicine, blood transfusion research center, Iran, tehran university of medical sciences, school of advanced technologies in medicine, department of medical biotechnology, Iran, academic center for education, culture and research (acecr), monoclonal antibody research center, avicenna research institute, Iran
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پست الکترونیکی
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rabbani@ari.ir
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Authors
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